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KMID : 0380020100250050459
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Korean Journal of Biotechnology and Bioengineering
2010 Volume.25 No. 5 p.459 ~ p.465
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Simultaneous Determination of Tar Color Additives in Cold Syrups by Ion Pair-high Performance Liquid Chromatography
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Jin Jing-Yu
Huang Hu
Lee Beom-Gyu
Lee Won-Jae
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Abstract
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A simple and efficient analytical method for the simultaneous determination of seven tar color additives was developed using ion pair high performance liquid chromatography. The conditions for HPLC analysis were as follows: column, μ-Bondapak C18 (10 μm, 300 × 3.9 mm i.d.); gradient mobile phase, 0.025 mol/L ammonium acetate (containing 0.01 mol/L tetrabutylammonium bromide)-acetonitrile-methanol (65：25：10) as a mobile for fraction A and 0.025 mol/L ammonium acetate (containing 0.01 mol/L tetrabutylammonium bromide)-acetonitrilemethanol (40：50：10) as a mobile for fraction B; flow rate, 1.0 mL/ min; detection wavelength, 254/520/620 nm. We could attain to the detection limits as 0.01~0.05 μg/mL (254 nm) and 0.005~0.01 μg/mL (520 nm) for six red tar color additives, and 0.05 μg/mL (254 nm) and 0.002 μg/mL (620 nm) for Fast green FCF. This analytical method was applicable to determine the tar color additives contained in several commercial cold syrups.
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KEYWORD
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Tar color additives, ion-pair high performance liquid chromatography, simultaneous determination, cold syrups
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